primary antibodies for birc5  (Boster Bio)

Journal: Translational Andrology and Urology

Article Title: Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer

doi: 10.21037/tau-24-359

Figure Lengend Snippet: Association between the expression of BIRC5 and HMMR genes and cancer prognosis. (A) Venn diagram revealing the identification of 159 DEGs at the intersection among three subgroups of PCa. (B) KEGG pathway enrichment analysis results for the 159 DEGs. (C) Cytoscape MCODE module used to screen 10 hub-key genes. (D,E) Kaplan-Meier curves of BCRFS for BIRC5 and HMMR in TCGA-PRAD. (F,G) Kaplan-Meier curves of BCRFS for BIRC5 and HMMR in GSE70769. (H,I) Univariate Cox regression analysis for differentially expressed hub-key genes in TCGA-PRAD and GSE70769. PCa, prostate cancer; mPCa, metastatic prostate cancer; CSPC, castration-sensitive prostate cancer; CRPC, castration-resistant prostate cancer; NEPC, neuroendocrine prostate cancer; ECM, extracellular matrix; BCRFS, biochemical recurrence-free survival; HR, hazard ratio; TCGA, The Cancer Genome Atlas; PRAD, prostate adenocarcinoma; DEGs, differentially expressed genes; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Primary antibodies for BIRC5 (Survivin, dilution 1:100, A00379, Boster, Wuhan, China) and HMMR (dilution 1:100, PA12592, Boster, Wuhan, China) were applied and incubated overnight at 4 °C.

Techniques: Expressing

Journal: Translational Andrology and Urology

Article Title: Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer

doi: 10.21037/tau-24-359

Figure Lengend Snippet: Analysis of the expression levels of BIRC5 and HMMR. (A) BIRC5 expression in tumor and normal tissues from TCGA pan-cancer and GTEx data. (B) HMMR expression in tumor and normal tissues from TCGA pan-cancer and GTEx data. (C,D) BIRC5 and HMMR expression in mPCa, localized PCa, and benign tissues from GSE3325. (E,F) BIRC5 and HMMR expression in NEPC and CSPC/CRPC tissues from GSE59984. (G) BIRC5 expression in mCRPC and localized PCa tissues from GSE35988. (H) HMMR expression in mCRPC and localized PCa tissues from GSE32269. *, P<0.05; **, P<0.01; ***, P<0.001; ns, not significant. TPM, transcripts per million; PCa, prostate cancer; mPCa, metastatic prostate cancer; CSPC, castration-sensitive prostate cancer; CRPC, castration-resistant prostate cancer; NEPC, neuroendocrine prostate cancer; PC, principal component; mCRPC, metastatic castration-resistant prostate cancer; TCGA, The Cancer Genome Atlas; GTEx, Genotype-Tissue Expression.

Article Snippet: Primary antibodies for BIRC5 (Survivin, dilution 1:100, A00379, Boster, Wuhan, China) and HMMR (dilution 1:100, PA12592, Boster, Wuhan, China) were applied and incubated overnight at 4 °C.

Techniques: Expressing

Journal: Translational Andrology and Urology

Article Title: Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer

doi: 10.21037/tau-24-359

Figure Lengend Snippet: Correlation and enrichment analyses of BIRC5 and HMMR. (A,B) Correlation between BIRC5, HMMR, and various immune cells; (C-H) association between the expression levels of BIRC5, HMMR, and the enrichment scores of various immune cells. *, P<0.05; **, P<0.01; ***, P<0.001.

Article Snippet: Primary antibodies for BIRC5 (Survivin, dilution 1:100, A00379, Boster, Wuhan, China) and HMMR (dilution 1:100, PA12592, Boster, Wuhan, China) were applied and incubated overnight at 4 °C.

Techniques: Expressing

Journal: Translational Andrology and Urology

Article Title: Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer

doi: 10.21037/tau-24-359

Figure Lengend Snippet: Correlation analysis between BIRC5 and HMMR expression and T helper cell and CD4 + /CD8 + T cell infiltration. (A-C) Correlation between BIRC5 expression in distinct PCa subgroups and Th cell infiltration levels using the xCELL algorithm. (D-F) Correlation between HMMR expression in distinct PCa subgroups and Th cell infiltration levels using the xCELL algorithm. (G-I) Correlation between BIRC5 expression in distinct PCa subgroups and CD4 + /CD8 + T cell infiltration levels using the quanTIseq algorithm. (J-L) Correlation between HMMR expression in distinct PCa subgroups and CD4 + /CD8 + T cell infiltration levels using the quanTIseq algorithm. PCa, prostate cancer; mCRPC, metastatic castration-resistant PCa; NEPC, neuroendocrine prostate cancer.

Article Snippet: Primary antibodies for BIRC5 (Survivin, dilution 1:100, A00379, Boster, Wuhan, China) and HMMR (dilution 1:100, PA12592, Boster, Wuhan, China) were applied and incubated overnight at 4 °C.

Techniques: Expressing

Journal: Translational Andrology and Urology

Article Title: Identification of BIRC5 and HMMR as prognostic biomarkers for immune infiltration in prostate cancer

doi: 10.21037/tau-24-359

Figure Lengend Snippet: Protein expression of BIRC5 and HMMR in PCa. (A-F) IHC images demonstrating the staining intensity of BIRC5 and HMMR in normal prostate tissues, Gleason ≤7 group, and Gleason >7 group (400×). (G,H) The AOD of IHC images of BIRC5 and HMMR used for comparisons among normal prostate tissues, the Gleason ≤7 group, and the Gleason >7 group. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001; AOD, average optical density; IHC, immunohistochemistry.

Article Snippet: Primary antibodies for BIRC5 (Survivin, dilution 1:100, A00379, Boster, Wuhan, China) and HMMR (dilution 1:100, PA12592, Boster, Wuhan, China) were applied and incubated overnight at 4 °C.

Techniques: Expressing, Staining, Immunohistochemistry

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Lists of DEGs upregulated/downregulated in dataset GSE112790

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: GO-BP and KEGG pathway analyses of upregulated DEGs associated with autophagy

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques: Phospho-proteomics, Modification, Shear

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Information on differentially regulated genes

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Expression levels of IGF1, CDKN2A, BIRC 5, and SPP1 in HCC from the GEO and TCGA databases. A – H IGF1, CDKN2A, BIRC5, and SPP1 mRNA expression levels are based on the TCGA database, including paired ( A – D ) and nonpaired samples ( E – H ). I – L The mRNA expression levels of IGF1, CDKN2A, BIRC5, and SPP1 mRNA are based on GSE84402

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Levels of IGF1, CDKN2A, BIRC5, and SPP1 in normal individuals and patients with HCC from the HPA database

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Analysis of the clinical implications of IGF1, CDKN2A, BIRC5, and SPP1 on the XIANTAO planform. A – D ROC curve analysis for IGF1, CDKN2A, BIRC5, and SPP1. E – H Overall survival analysis for IGF1, CDKN2A, BIRC5, and SPP1. I – L Analysis of the association of TNM stages with IGF1, CDKN2A, BIRC5, and SPP1

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: GSEA for IGF1, CDKN2A, BIRC5, and SPP1 by the TCGA database

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Levels of IGF1, CDKN2A, BIRC5, SPP1 and their correlations with the TME on the XIANTAO planform. A – D Immune infiltration analysis for IGF1, CDKN2A, BIRC5 and SPP1. E – H Correlation levels of IGF1, CDKN2A, BIRC5, and SPP1 with the level of immune infiltration

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Immune infiltration analysis of IGF1, CDKN2A, BIRC5, and SPP1 performed using the TIMER database

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Immune analysis of IGF1, CDKN2A, BIRC5, and SPP1 by the TCGA database. A – D Correlations of the estimated proportions of immune and stromal cells with IGF1, CDKN2A, BIRC5, and SPP1 levels in HCC. E Various immune checkpoints were associated with IGF1, CDKN2A, BIRC5, and SPP1

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques:

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Levels of IGF1, CDKN2A, BIRC5, and SPP1 in patient tissues by IHC. Levels of CDKN2A, BIRC5, and SPP1 in HCC were increased compared with tumor-adjacent tissues. However, IGF1 expression was downregulated in HCC tissues compared with adjacent tissues

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques: Expressing

Journal: Discover Oncology

Article Title: Autophagy-related biomarkers in hepatocellular carcinoma and their relationship with immune infiltration

doi: 10.1007/s12672-024-01167-x

Figure Lengend Snippet: Expressions of IGF1, CDKN2A, BIRC5, and SPP1 in patient tissues by RT‒PCR. The expressions of CDKN2A, SPP1, and BIRC5 in HCC were increased compared with tumor-adjacent tissues. However, IGF1 expression was downregulated in HCC tissues compared with that in adjacent tissues

Article Snippet: After blocking with a mixture of methanol and 0.75% hydrogen peroxide, sections were incubated with anti-IGF1 primary antibody (D160510, Sangon Biotech, China; dilution 1:50), anti-CDKN2A primary antibody (D227441, Sangon Biotech, China; dilution 1:65), anti-BIRC5 primary antibody (D120314, Sangon Biotech, China; dilution 1:60) and anti-SPP1 antibody (D121078, Sangon Biotech, China; dilution 1:20) at 4 °C overnight.

Techniques: Expressing

Journal: International journal of molecular sciences

Article Title: BIRC5 Inhibition Is Associated with Pyroptotic Cell Death via Caspase3-GSDME Pathway in Lung Adenocarcinoma Cells.

doi: 10.3390/ijms241914663

Figure Lengend Snippet: Figure 3. Validation of the key hub genes. (a) Heat map of eight hub genes. (b) The qRT-PCR analysis of eight candidate genes in human lung adenocarcinoma and peri-tumor samples. Values of * p < 0.05 and *** p < 0.001 vs. peri-tumor (n = 5). (c) Representative immunohistochemistry images of BIRC5 from normal lung tissue and LUAD tissues extracted from the Human Protein Atlas database. (d) The protein level of BIRC5 in mouse orthotopic LUAD and adjacent normal tissue, value of ** p < 0.01 vs. peri-tumor (n = 6). (e) The protein level of BIRC5 in human LUAD and peri-tumor samples, value of ** p < 0.01 vs. peri-tumor (n = 3).

Article Snippet: The sections were incubated with BIRC5 primary antibody (1:100; #2808S, Cell Signaling Technology, USA) overnight at 4 °C, followed by 2 h incubation with the corresponding horseradish peroxidase-conjugated secondary antibody.

Techniques: Biomarker Discovery, Quantitative RT-PCR, Immunohistochemistry

Journal: International journal of molecular sciences

Article Title: BIRC5 Inhibition Is Associated with Pyroptotic Cell Death via Caspase3-GSDME Pathway in Lung Adenocarcinoma Cells.

doi: 10.3390/ijms241914663

Figure Lengend Snippet: Figure 6. The BIRC5 deletion inhibits LUAD progression. (a) Western blot analysis of BIRC5 after transfection of BIRC5 siRNA into A549 cell, value of * p < 0.05 vs. si-NC (n = 3). (b) Representative micro-PET/CT images of tumor region after intravenous injection of 18F-FDG (n = 3). (c) Images of A549 xenografts tumor (n = 5). (d) Tumor volume of A549 xenografts tumor in each group, value of ** p < 0.01 vs. si-NC (n = 5). (e) Body weight of A549 xenografts tumor in each group, value of ** p < 0.01 vs. si-NC (n = 5). (f) Cell viability of BIRC5 inhibition measured with CCK-8 assay, value of ** p < 0.01 vs. si-NC (n = 3). (g) The cell migration was measured using transwell assay, value of * p < 0.05 vs. si-NC (n = 3).

Article Snippet: The sections were incubated with BIRC5 primary antibody (1:100; #2808S, Cell Signaling Technology, USA) overnight at 4 °C, followed by 2 h incubation with the corresponding horseradish peroxidase-conjugated secondary antibody.

Techniques: Western Blot, Transfection, Micro-PET, Injection, Inhibition, CCK-8 Assay, Migration, Transwell Assay

Journal: International journal of molecular sciences

Article Title: BIRC5 Inhibition Is Associated with Pyroptotic Cell Death via Caspase3-GSDME Pathway in Lung Adenocarcinoma Cells.

doi: 10.3390/ijms241914663

Figure Lengend Snippet: Figure 7. BIRC5 bond with Caspase3. (a) Docking analysis of the interaction between BIRC5 (green) and caspase3 (rose red). (b) Co-immunoprecipitation (Co-IP) validation of interaction between BIRC5 and caspase3 (n = 3). (c) The relative protein levels of caspase3 (n = 4). (d) The relative expression levels of cleaved caspase3 protein n = 3, value of ** p < 0.01 vs. si-NC, (n = 4). (e) The percentage of PI+ cells and Annexin V−FITC+ and PI−were analyzed with flow cytometry, value of ** p < 0.01 vs. si-NC (n = 3).

Article Snippet: The sections were incubated with BIRC5 primary antibody (1:100; #2808S, Cell Signaling Technology, USA) overnight at 4 °C, followed by 2 h incubation with the corresponding horseradish peroxidase-conjugated secondary antibody.

Techniques: Immunoprecipitation, Co-Immunoprecipitation Assay, Biomarker Discovery, Expressing, Cytometry

Journal: International journal of molecular sciences

Article Title: BIRC5 Inhibition Is Associated with Pyroptotic Cell Death via Caspase3-GSDME Pathway in Lung Adenocarcinoma Cells.

doi: 10.3390/ijms241914663

Figure Lengend Snippet: Figure 8. BIRC5 mediated apoptosis to pyroptosis. (a) The LDH content in the culture supernatants of A549 cells transfected with BIRC5 siRNA, value of ** p < 0.01 vs. si-NC (n = 5). (b) The relative expression levels of GSDMD, GSDMD-N, caspase1, cleaved caspase1, GSDME and GSDME-N in A549 cells transfected with BIRC5 siRNA using Western blot, value of ** p < 0.01 vs. si-NC (n ≥3). (c) The percentage of PI+ cells and Annexin V-FITC+ and PI−in each group were analyzed with flow cytometry, values of * p < 0.05, ** p < 0.01, ## p < 0.01 (n = 3), respectively. ns, not significant.

Article Snippet: The sections were incubated with BIRC5 primary antibody (1:100; #2808S, Cell Signaling Technology, USA) overnight at 4 °C, followed by 2 h incubation with the corresponding horseradish peroxidase-conjugated secondary antibody.

Techniques: Transfection, Expressing, Western Blot, Cytometry